Part:BBa_K1413021

bphR2 mutated

bphR2 gene, from Pseudomonas pseudoalcaligenes KF707, encodes for a regulatory protein (bphR2) which can detect polychlorinated biphenyls (PCBs). It is involved in the positive regulation of bphr1 gene. In the absence of biphenyl, small amounts of bphR2 protein binds to bphR2 operator to repress bphR2 transcription and activate bphR1 weakly. When there is biphenyl in the media, bphR2 protein binds to bphR1 to activate strongly its transcription. This allows to initiate the degradation of biphenyl.

Our aim was to finish and characterize the part of 2013 Paris Saclay's team who wanted to do a PCB biosensor with the system bphR2/bphR1. We used bphR2 in our biosensor project of PCBs. We have a fluorescence system with RFP (BBa_E1010) to detect compound.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 475
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 259