Part:BBa_K1412089
Riboregulator which combines crRNA and RBS acting as a lock to the gene cuicuit
This part is based on the Callra J M et al’s (1)riboregulator crRNA. In order to use our project "aptamer key" (BBa_B1412088)to open the lock, we use AGGAGGAAA as our RBS. Here we add BBa_B01060,which is promoter tetR to transcrip our DNA.
Usage and Biology
The Callra J M et al's work is the continuation of the work done by Isaacs et al(2). From the paper, we notice that we can add aptamer before taRNA to regulate taRNA express, which in turn influence the expression of the crRNA. In our project, we consider our lock can be opened by different RNA aptamer if the aptamer construct the taRNA in the same way according to our project.
When this part was transcript into mRNA, it forms a stem-loop structure which “locks” the ribosome binding site. This folded mRNA structure is known as the “ cis-repressed” part. If BBa_K1412088 available, when we add theophylline into the medium, the taRNA can exposes, which destabilize the stem-loop enough to allow the corresponding taRNA to interject crRNA and open the loop.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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