Coding

Part:BBa_K1408001

Designed by: Stephen A Rettie   Group: iGEM14_Washington   (2014-10-09)


Gal4-Vp16 Transcriptional Activator

Gal4 protein binds to a Gal1 promoter while VP16 recruits transcription machinery promoting transcription to anything under the Gal1 promoter. The two must be co-localized to work as a transcriptional activator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 338
    Illegal XhoI site found at 83
    Illegal XhoI site found at 566
    Illegal XhoI site found at 1105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 485


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Categories
Parameters
None