Composite

Part:BBa_K1407016

Designed by: Ye Chen   Group: iGEM14_BIT   (2014-10-10)


LasR+LasR/PAI1 Inducible promoter+GFP+RhlR(LVA-)+RhlR/PAI2 Inducible promoter+RFP

It's composed of seven sections:constitutive production of LasR,LasR/PAI1 Inducible promoter with RBS(B0034)and GFP,terminator,constitutive promoter,RhlR protein generator(LVA-) ,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,Terminator.When PAI1 exsits,the part could be inducibled and expresses GFP protein;When PAI2 exsits,the part could be inducibled and expresses RFP protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 987
    Illegal NheI site found at 1984
    Illegal NheI site found at 2007
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
    Illegal AgeI site found at 3549
    Illegal AgeI site found at 3661
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2753
    Illegal BsaI.rc site found at 1756


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Categories
Parameters
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