Composite

Part:BBa_K1407013

Designed by: Ye Chen   Group: iGEM14_BIT   (2014-10-10)


LasR+LasR/PAI1 Inducible promoter+RFP+RhlR+RhlR/PAI2 Inducible promoter+RFP

It's composed of seven sections:constitutive production of LasR,LasR/PAI1 Inducible promoter with RBS(B0030)and RFP,terminator,constitutive promoter,RhlR protein generator ,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,Terminator.When PAI2/PAI1 exsits,the part could be inducibled and expresses RFP protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 987
    Illegal NheI site found at 1973
    Illegal NheI site found at 1996
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2267
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
    Illegal AgeI site found at 1670
    Illegal AgeI site found at 1782
    Illegal AgeI site found at 3596
    Illegal AgeI site found at 3708
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2742


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Categories
Parameters
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