Composite

Part:BBa_K1407012

Designed by: Ye Chen   Group: iGEM14_BIT   (2014-10-10)


constitutive promoter+RhlR protein generator(LVA-)+RhlR/PAI2 Inducible promoter+RBS(B0030)+RFP+Termi

It's composed of four sections:constitutive promoter,RhlR protein generator(LVA-) ,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,Terminator.By adding PAI2,the part could be inducibled and expresses RFP protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1572
    Illegal AgeI site found at 1684
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 776


[edit]
Categories
Parameters
None