Composite

Part:BBa_K1407010

Designed by: Ye Chen   Group: iGEM14_BIT   (2014-10-10)


constitutive production of LasR+LasR/PAI1 Inducible promoter +RBS(B0030)+RFP+Terminator

It's composed of three sections:constitutive production of LasR,LasR/PAI1 inducible promoter with RBS (B0030) and RFP,Terminator.By adding PAI1,the part could be inducibled and expresses RFP protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 987
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
    Illegal AgeI site found at 1670
    Illegal AgeI site found at 1782
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None