Composite

Part:BBa_K1407007

Designed by: Ye Chen   Group: iGEM14_BIT   (2014-10-10)


RhlR protein(LVA-) +RhlR/PAI2 Inducible promoter +RBS(B0030)+RFP+Terminator

It's composed of three sections:RhlR CDS (no LVA tag) and terminator,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,terminator.With the RhlR protein existing,by adding PAI2,the part could be inducibled and expresses RFP protein.Adding a promoter before the RhlR as you want.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 258
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1529
    Illegal AgeI site found at 1641
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 733


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Parameters
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