Composite

Part:BBa_K1407006

Designed by: Ye Chen   Group: iGEM14_BIT   (2014-10-10)


constitutive production of LasR + LasR/PAI1 Inducible promoter +RBS(B0034)+GFP

It's composed of two sections:constitutive production of LasR,LasR/PAI1 inducible promoter with RBS (B0034) and GFP.With the RhlR protein existing,by adding PAI2,the part could be inducibled and expresses RFP protein.Before using it,you'd better add a terminator to the part's tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 987
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1756


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Categories
Parameters
None