Composite

Part:BBa_K1407002

Designed by: Ye Chen   Group: iGEM14_BIT   (2014-10-10)

RhlR(LVA+)+RhlR/PAI2 Inducible promoter +RBS(B0030)+RFP

it's composed of two sections: RBS(B0034)+RhlR(LVA+)+RhlR/PAI2 inducible promoter with RBS (B0030) and RFP. With the RhlR promoter existing,by adding PAI2,the part could be inducible and expresses RFP promoter.Before use it,you'd better add a terminator to the part's tag.And add a promoter before the RhlR as you want.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 258
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1587
    Illegal AgeI site found at 1699
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 733


[edit]
Categories
Parameters
None