Composite

Part:BBa_K1406001

Designed by: Sierra Tackett   Group: iGEM14_WLC-Milwaukee   (2014-10-09)


pLAC promoter + strong RBS

A combinatorial part of R0011, B0034, and a NheI cutsite. This allows a in-frame, orientated insertion of a biobrick part behind the pLac promoter and the strong RBS using the restriction enzymes NheI and PstI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 116
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None