Part:BBa_K1405009

INPNC-ModA

Introduction

modA a periplasmic binding protein, so we should link a outer membrane protein, Ice-nucleation protein (INP) to let it stay locate at the outer membrane surface. So we use a linker to link two parts for location. The BBa_K1405005 contains INPN, ModA and a linker.

INP has been successfully used to display several proteins, such as levansucrase, carboxymethylcellulase (CMCase), Hepatitis B surface antigen (HbsAg), human immunodeficiency virus type 1 (HIV-1) gp120 and so on[1]. This was achieved using either full-length sequences or truncated portions containing only N- and C-domains (INP- NC) or INP-NC with five additional internal repeating units to display foreign protein on the surface of cell. containing only N- and C-domains means no repeat sequent, producing no ice-nucleation activity. This indicates that the central repeating domains are not required for export to the cell surface, and are therefore, ideal spacer units to control the distance between the passenger protein and the cell surface. Importantly, INP can be expressed at the cell surface of E. coli at a very high level, without affecting cell viability: comparable to the endogenous expression of the OmpA porin[2].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 409
  
• 1000
  COMPATIBLE WITH RFC[1000]

reference

[1] Mei Li Wu, Chun Yung Tsai& Tsai Hsia Chen, 2005, Cell surface display of Chi92 on Escherichia coliusing ice nucleation protein for improved catalytic and antifungal activity
[2] Edwin van Bloois, Remko T. Winter, Harald Kolmar and Marco W. Fraaije, 2010, Decorating microbes: surface display of proteins on Escherichia coli.