Coding

Part:BBa_K1405005

Designed by: Fengjing，Ningze Sun,Xuan Wang   Group: iGEM14_BNU-China   (2014-10-12)

INPN+ModA

Introduction

ModA a periplasmic binding protein, so we should link a outer membrane protein, Ice-nucleation protein (INP) to let it stay locate at the outer membrane surface. So we use a linker to link two parts for location. The BBa_K1405005 contains INPN, ModA and a linker. This plasmid is a composite part used in our molybdate binding assay. It used pGEX4T1 as the vector and expressed the protein and purified the protein by GST-affinity chromatography, and we designed a 2aa protein, its sequence can be cut by SalI, so we can replace different proteins easily. You could find more details.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 429
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 238


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