Part:BBa_K1405003

INPN

Introduction

Ice-nucleation protein (INP), an outer membrane protein from Pseudomonas syringae.INP has been successfully used to display several proteins, such as levansucrase, carboxymethylcellulase (CMCase).Functional truncated INP molecules may serve as better anchoring motifs to carry large heterologous proteins,such as INPN. Then we found that using INP derivatives containing only N-domain (INPN) as display system successfully is possible. The passenger proteins are also various, such as Japanese Encephalitis Virus Pathogenicity, phosphate-binding protein and so on. There is also literature which alleges that since full-length INP is quite large (1,200–1,500 amino acid residues), functional truncated INP may carry larger proteins with less burden.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 429
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 238
  

Characterization

INPN can help us display proteins on the outer membrane of E. coli cells. We hope that we can use truncated inaK, which encodes inpn, as target gene of recombinant plasmid to alleviate burden of E.coli. May carry larger passerger protein than full-length INP.