Paris Bettencourt 2014 Odor Palette
Our odor palette is composed of BioBricks containing codon-optimized sequences of enzymes known to catalyze reactions that yield volatile compounds with distinctive smells.
We included odors with different tonalities in order to explore the aromas resulting from combinations of smelly units in E. coli. The tonality of each smell is categorized as butter, balminess, citrus, non-citrus fruit and herbal. These cover half of the main odor categories perceivable to human beings.
Each part has a standardized architecture consisting on a constitutive promoter and a functional RBS. The palette could be used as an educational tool to introduce synthetic biology concepts to the general public.
|JMT||Jasmonic acid||1 mM||Methyl jasmonate|
|BMST1||Salicylic acid||2 mM||Methyl salicylate|
|ATF1||Isoamyl alcohol||5 mM||Isoamyl acetate|
|BMST1||Benzoic acid||5 mM||Methyl benzoate|
BBa_K1403017 & BBa_K1403019
|agaA||3-M-2-hexenoic acid||-||3-HO-3-Methylhexanoic acid|
Acetohydroxybutanoate synthase /acetolactate synthase (ilvB/N) expression cassette
- Constitutive T7 promoter
- CDS: IlvB/N from E. coli MG1655
Transform E. coli to overexpress ilvB and thus increase the basal production of alpha acetolactate, the precursor of acetoin.
- This was submitted in a pETDuet-1
Click on Design tab for more information.
Sequence and Features
- 10Illegal XbaI site found at 47
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23Illegal XbaI site found at 47
- 25Illegal XbaI site found at 47
- 1000COMPATIBLE WITH RFC