Composite

Part:BBa_K1403018

Designed by: Cristina Garcia-Timermans   Group: iGEM14_aris_Bettencourt   (2014-10-06)


Expression of agaA in E coli

Name: BBa_K1403018 Description: Biobrick submission of agaA expression construct Resistances : Cm Parts: Promoter - RBS - agaA - his tag Design date: 11/June/2014


agaA is a gene present in Corynebacterium striatum and it is the main responsible for body odor according to the literature. It transforms 3-methyl-2-hexenoic acid into 3-hydroxy-3-methylhexanoic acid.

Detailed description of the agaA cosntruct: - Promoter BBa_J23108 from the Anderson's promoter collection

- RBS from the RBS Calculator of Salis lab specific for E coli

- BioBrick Prefix + Scar from the iGEM Parts webpage

- agaA sequence codon optimized for E coli 12 (IDT online tool). We checked with Generious that there were no restriction sites for EcoRI, SpeI, XbaI and PstI.

- Histidine tag: 6 Histidines in C-terminal were added -Stop codon -BioBrick scar + BioBrick suffix from the iGEM Parts webpage

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 372
    Illegal BamHI site found at 603
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 265
  • 1000
    COMPATIBLE WITH RFC[1000]


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