Part:BBa_K1403017
Paris Bettencourt 2014 Odor Palette
Our odor palette is composed of BioBricks containing codon-optimized sequences of enzymes known to catalyze reactions that yield volatile compounds with distinctive smells.
We included odors with different tonalities in order to explore the aromas resulting from combinations of smelly units in E. coli. The tonality of each smell is categorized as butter, balminess, citrus, non-citrus fruit and herbal. These cover half of the main odor categories perceivable to human beings.
Each part has a standardized architecture consisting on a constitutive promoter and a functional RBS. The palette could be used as an educational tool to introduce synthetic biology concepts to the general public.
| System | Enzyme | Substrate | Concentration | Product |
|---|---|---|---|---|
| Jasmine BBa_K1403006 | JMT | Jasmonic acid | 1 mM | Methyl jasmonate |
| Mint BBa_K1403009 | BMST1 | Salicylic acid | 2 mM | Methyl salicylate |
| Banana BBa_K1403012 | ATF1 | Isoamyl alcohol | 5 mM | Isoamyl acetate |
| Limonene BBa_K1403003 | LIMS1 | Farnesyl diphosphate | Natural | (+)-Limonene |
| Rain BBa_K1403013 | GDS | Farnesyl diphosphate | Natural | Geosmin |
| Flowers BBa_K1403009 | BMST1 | Benzoic acid | 5 mM | Methyl benzoate |
| Butter BBa_K1403017 & BBa_K1403019 | AldB ilvB/N | Acetolactic acid Pyruvate | Natural | Acetoin |
| Sweat BBa_K1403002 | agaA | 3-M-2-hexenoic acid | - | 3-HO-3-Methylhexanoic acid |
Acetaldehyde dehydrogenase (aldB) expression cassette
- Constitutive promoter BBa_J23108
- Synthetic RBS
- CDS: aldB from Lactococcus lactis
- Terminators BBa_B0010 & BBa_B0012
Transform E. coli to produce acetoin.
AldB requires alpha acetolactate as substrate.
A co-transformation with part BBa_K1403019 increases the amount of intracellular alpha acetolactate.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 71
- 1000COMPATIBLE WITH RFC[1000]
Click on Design tab for more information.
Verification
Expeceted bands at 2kbp for pSB1C3 and 846bp for aldB gene with promoter
| None |