Paris Bettencourt 2014 Odor Palette
Our odor palette is composed of BioBricks containing codon-optimized sequences of enzymes known to catalyze reactions that yield volatile compounds with distinctive smells.
We included odors with different tonalities in order to explore the aromas resulting from combinations of smelly units in E. coli. The tonality of each smell is categorized as butter, balminess, citrus, non-citrus fruit and herbal. These cover half of the main odor categories perceivable to human beings.
Each part has a standardized architecture consisting on a constitutive promoter and a functional RBS. The palette could be used as an educational tool to introduce synthetic biology concepts to the general public.
|JMT||Jasmonic acid||1 mM||Methyl jasmonate|
|BMST1||Salicylic acid||2 mM||Methyl salicylate|
|ATF1||Isoamyl alcohol||5 mM||Isoamyl acetate|
|BMST1||Benzoic acid||5 mM||Methyl benzoate|
BBa_K1403017 & BBa_K1403019
|agaA||3-M-2-hexenoic acid||-||3-HO-3-Methylhexanoic acid|
Benzoic acid/salicylic acid carboxyl methyltransferase 1 (BSMT1) expression cassette
- Constitutive promoter BBa_J23108
- Synthetic RBS
- CDS: BSMT1 from Petunia × hybrida
- Histidine Tag
Transform E. coli and grow in the presence of salicylic acid or benzoic acid for production of methyl salycilate or methyl benzoate respectively.
Click on Design tab for more information.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 71
- 1000COMPATIBLE WITH RFC