Paris Bettencourt 2014 Odor Palette
Our odor palette is composed of BioBricks containing codon-optimized sequences of enzymes known to catalyze reactions that yield volatile compounds with distinctive smells.
We included odors with different tonalities in order to explore the aromas resulting from combinations of smelly units in E. coli. The tonality of each smell is categorized as butter, balminess, citrus, non-citrus fruit and herbal. These cover half of the main odor categories perceivable to human beings.
Each part has a standardized architecture consisting on a constitutive promoter and a functional RBS. The palette could be used as an educational tool to introduce synthetic biology concepts to the general public.
|JMT||Jasmonic acid||1 mM||Methyl jasmonate|
|BMST1||Salicylic acid||2 mM||Methyl salicylate|
|ATF1||Isoamyl alcohol||5 mM||Isoamyl acetate|
|BMST1||Benzoic acid||5 mM||Methyl benzoate|
BBa_K1403017 & BBa_K1403019
|agaA||3-M-2-hexenoic acid||-||3-HO-3-Methylhexanoic acid|
Limonene synthase (LIMS1) expression cassette
- Constitutive promoter BBa_J23108
- Synthetic RBS
- CDS: LIMS1 from Citrus limon
Transform E. coli for (+)-Limonene production.
Click on Design tab for more information.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12INCOMPATIBLE WITH RFCIllegal NheI site found at 7
Illegal NheI site found at 30
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25INCOMPATIBLE WITH RFCIllegal AgeI site found at 82
- 1000INCOMPATIBLE WITH RFCIllegal SapI.rc site found at 187
Expected bands at 2kbp for pSB1C3 and 1798bp for the limonene generator