Part:BBa_K1402014

BTE-CDS

Acyl carrier protein (ACP) thioesterase catalyzes the hydrolysis of acyl-ACP into fatty acid and ACP. This reaction removes the coupling between fatty acid biosynthesis and phospholipid biosyntheis, which is a key to obtaining free fatty acids. This thioesterase BTE can produce fatty acids with 12~14 carbon chain length. In previous reserches, thioesterase gene BTE can be expressed in E.coli BL21(DE3) and enhance the synthesis of free fatty acids.This part can increase the productivity of free fatty acids. However,this part can not express itself. You should add promoter, RBS and terminator to it or using other parts we offer.

In the mixture, we obtained 5 kinds of fatty acid, namely fatty acid of 10C chain length, 12C chain length, 14C chain length, 16C chain length and 18C chain length. Among them, fatty acid of 12C chain length accounts for the greatest amount of 74.2% and fatty acid of 10C chain length is the least amount of 1%. Proportion of fatty acid of 14C, 16C and 18C is 12.9%, 8% and 3.9% respectively.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 484
  Illegal BglII site found at 624
  Illegal BamHI site found at 73
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1033