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Part:BBa_K1402013

Designed by: Chen Xi   Group: iGEM14_NJU-QIBEBT   (2014-09-29)


FadD-RFP-FabB-GFP

FabB promoter and FabD promoter reacts differently to the changing in concentration of fatty acids. We activate the expression of GFP and RFP by FabB promoter and FadD promoter respectively. When fatty acid concentration fluctuates, the changing in the expression of GFP and RFP tells us the fluctuation of fatty acid concentration. This part functions as a biosensor. In the abundance of fatty acid, the red fluorescence is bright. On the contrary, in the absence of fatty acid, the green fluorescence is bright.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1499
    Illegal AgeI site found at 1611
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 730


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