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Part:BBa_K1402012

Designed by: Chen Xi   Group: iGEM14_NJU-QIBEBT   (2014-09-29)


FadD-RBS-RFP

This part is sensitive to long chain acyl-CoA ,with a FadR binding site. In the absence of long chain acyl-CoA, FadR represses the transcription of RFP. In the presence of long chain acyl-CoA, FadR dissociates from the binding site so that the transcription of RFP begins. This part can be used to test the function of FadD. It can also indicates the relative concentration of fatty acid. If you want to use this promoter, choose another part we offer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 685
    Illegal AgeI site found at 797
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None