Reporter

Part:BBa_K1401616

Designed by: Yash Agarwal   Group: iGEM14_BostonU   (2014-10-11)


pLac - BCD2 - tetR- GFP

The parts contain the BioBricks - R0010_EB, BCD2_BC, C0040_CI, E0040m_ID and B0015_DF. All of these are MoClo variants i.e. they have MoClo flanking regions attached to each basic part. A new fusion site (I) was specifically designed to create the fusion protein (regulator + reporter).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1010
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1010
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1010
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None