Coding

Part:BBa_K1401002

Designed by: Kathleen Lewis   Group: iGEM14_BostonU   (2014-07-21)


MoClo Level 0 part of SrpR Repressible gene. TetR homolog

This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: C = AATG; D = AGGT

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 179
    Illegal PstI site found at 392
    Illegal PstI site found at 458
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 179
    Illegal PstI site found at 392
    Illegal PstI site found at 458
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 179
    Illegal PstI site found at 392
    Illegal PstI site found at 458
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 179
    Illegal PstI site found at 392
    Illegal PstI site found at 458
    Illegal AgeI site found at 494
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None