Reporter

Part:BBa_K1399022

Designed by: Anna Stikane   Group: iGEM14_Edinburgh   (2014-09-19)

Ptet-GFP(DAS+2)

Tetracycline/aTc inducible promoter with GFP reporter tagged with NYADAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724


[edit]
Categories
//cds/reporter/gfp
//classic/generator/prct
//classic/reporter/pret
//function/reporter/fluorescence
Parameters
emissionGFP
excitation
tagSsrA-DAS+2 degradation tag