Reporter

Part:BBa_K1399019

Designed by: Anna Stikane   Group: iGEM14_Edinburgh   (2014-09-19)


Ptet-GFP(AAV)

Tetracycline/aTc inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724


[edit]
Categories
//cds/reporter/gfp
//classic/generator/prct
//classic/reporter/pret
//function/reporter/fluorescence
Parameters
emissionGFP
excitation
tagSsrA-AAV degradation tag