Composite

Part:BBa_K1398001

Designed by: Exeter iGEM 2014   Group: iGEM14_Exeter   (2014-08-11)

XenB (Inducible Construct)

A construct created to degrade TNT and nitroglycerin. The construct contains the coding sequence for XenB (BBa_K1398000), an enzyme with the capability to degrade nitro- groups in chemicals. The construct also contains a Lactose inducible promoter (BBa_R0010), a strong RBS (BBa_B0034) and a double terminator of BBa_B0010 and BBa_B0012. The protein has been codon-optimised for expression in E. coli by DNA2.0. More information can be found here: https://www.dna20.com/services/genegps.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//function/degradation
Parameters
None