Composite

Part:BBa_K1395003

Designed by: Gaurav Sinsinbar,Nikhil Patidar ,Chandel Angad Singh   Group: iGEM14_IIT_Delhi   (2014-10-10)

sqr gene (sulfide quinone reductase) and cysI gene (sulfur reductase) under constitutive promoter

This part is composite biobrick having parts of biobricks Bba_K896000 and Bba_K896001. The first part is sqr gene obtained from biobrick Bba_K896000. In this part the gene is expressed under a constitutive promoter and this enzyme converts the sulphide (S-2 ) to elemental Sulfur. And the second part is cysI gene obtained from Bba_K896001 which converts sulphite(SO32-) to sulphide (S-2 ) . So, these two parts codes for proteins which simultaneously work to convert sulphite (SO32-)to elemental Sulfur.

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Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1997
    Illegal PstI site found at 1463
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1997
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 1463
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1997
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1997
    Illegal PstI site found at 1463
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1997
    Illegal PstI site found at 1463
    Illegal NgoMIV site found at 1736
    Illegal NgoMIV site found at 1922
    Illegal NgoMIV site found at 2064
    Illegal AgeI site found at 1436
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 390
    Illegal BsaI site found at 1472
    Illegal BsaI site found at 2794


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