Composite

Part:BBa_K1392910

Designed by: Dorukcan Özköse   Group: iGEM14_METU_Turkey   (2014-10-10)


with L+ arabinose promoter, caterchol 2,3-dioxygenase activate to degration of catechol.

Catechol or catechol 2,3-dioxygenases (XylE) (C2,3O) + O(2) is converted by a ring cleavage into 2-hydroxymuconate semialdehyde which is the cytotoxic and bright yellow-coloured product[1]. This is a key enzyme in many (soil) bacterial species used for the degradation of aromatic compounds. Catechol 2,3-dioxygenase[2] was originally isolated from Pseudomonas putida and is active only as a homotetramer. With araC-Pbad promoter,the production of XylE start and bacteria degrade catechol to 2-hydroxymuconate semialdehyde.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1554
    Illegal NgoMIV site found at 1726
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2077
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None