Plasmid

Part:BBa_K1391143

Designed by: James Anderson   Group: iGEM14_MIT   (2014-10-30)


pEXPR Hef1a:BACE1

BACE1 miscleaves amyloid precursor protein (APP) at the beta-site. Subsequent cleavage of APP by gamma-secretase results in liberation of the cleaved fragment called beta-amyloid, one of the hallmark molecules in Alzheimer's pathology. This vector contains the gene for BACE1 under constitutive regulation by the Hef1a promoter. We utilized this vector in our experiment to test the effectiveness of our four miRNA-generating vectors at down-regulating BACE1 expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 5437
    Illegal EcoRI site found at 7028
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4564
    Illegal PstI site found at 5069
    Illegal PstI site found at 6126
    Illegal PstI site found at 6400
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 5437
    Illegal EcoRI site found at 7028
    Illegal NheI site found at 3210
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4564
    Illegal PstI site found at 5069
    Illegal PstI site found at 6126
    Illegal PstI site found at 6400
    Illegal NotI site found at 7870
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 5437
    Illegal EcoRI site found at 7028
    Illegal BglII site found at 4818
    Illegal BglII site found at 7108
    Illegal BamHI site found at 2854
    Illegal BamHI site found at 3328
    Illegal BamHI site found at 5424
    Illegal BamHI site found at 8065
    Illegal XhoI site found at 3538
    Illegal XhoI site found at 5217
    Illegal XhoI site found at 7850
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 5437
    Illegal EcoRI site found at 7028
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4564
    Illegal PstI site found at 5069
    Illegal PstI site found at 6126
    Illegal PstI site found at 6400
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 5437
    Illegal EcoRI site found at 7028
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4564
    Illegal PstI site found at 5069
    Illegal PstI site found at 6126
    Illegal PstI site found at 6400
    Illegal NgoMIV site found at 2297
    Illegal NgoMIV site found at 3741
    Illegal NgoMIV site found at 4024
    Illegal NgoMIV site found at 4952
    Illegal AgeI site found at 3456
    Illegal AgeI site found at 4330
    Illegal AgeI site found at 5477
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1121
    Illegal BsaI.rc site found at 5625
    Illegal SapI site found at 38
    Illegal SapI site found at 3214
    Illegal SapI site found at 8297
    Illegal SapI.rc site found at 5634


[edit]
Categories
Parameters
n/apEXPR Hef1a:BACE1