Plasmid

Part:BBa_K1391020

Designed by: Jing Wei "Raymond" Liu   Group: iGEM14_MIT   (2014-10-17)

MAV1212_hEF1a_eBFP2

eBFP2 fluorophore is constitutively expressed using a hEF1a promoter, within a MAV1212 backbone. Complete system for eBFP2 expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1085
    Illegal EcoRI site found at 2314
    Illegal EcoRI site found at 3387
    Illegal XbaI site found at 3360
    Illegal PstI site found at 730
    Illegal PstI site found at 1011
    Illegal PstI site found at 1440
    Illegal PstI site found at 1945
    Illegal PstI site found at 3348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1085
    Illegal EcoRI site found at 2314
    Illegal EcoRI site found at 3387
    Illegal PstI site found at 730
    Illegal PstI site found at 1011
    Illegal PstI site found at 1440
    Illegal PstI site found at 1945
    Illegal PstI site found at 3348
    Illegal NotI site found at 402
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1085
    Illegal EcoRI site found at 2314
    Illegal EcoRI site found at 3387
    Illegal BglII site found at 1694
    Illegal BglII site found at 3848
    Illegal BamHI site found at 3366
    Illegal XhoI site found at 394
    Illegal XhoI site found at 2093
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1085
    Illegal EcoRI site found at 2314
    Illegal EcoRI site found at 3387
    Illegal XbaI site found at 3360
    Illegal PstI site found at 730
    Illegal PstI site found at 1011
    Illegal PstI site found at 1440
    Illegal PstI site found at 1945
    Illegal PstI site found at 3348
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1085
    Illegal EcoRI site found at 2314
    Illegal EcoRI site found at 3387
    Illegal XbaI site found at 3360
    Illegal PstI site found at 730
    Illegal PstI site found at 1011
    Illegal PstI site found at 1440
    Illegal PstI site found at 1945
    Illegal PstI site found at 3348
    Illegal NgoMIV site found at 645
    Illegal NgoMIV site found at 926
    Illegal NgoMIV site found at 1828
    Illegal AgeI site found at 774
    Illegal AgeI site found at 1206
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3131
    Illegal BsaI.rc site found at 3749


A


B

C

Low Sensor miR-144 in the presence and absence of siRNA-144 in HEK293 cells. Using red as our transfection marker and blue as our output, we expected that the sensor, when exposed to the inhibiting siRNA, would show repressed eBFP2 output. However the cytometry results in figure (A) shows that there is no difference between the 'on' and 'off' state. This conclusion is reinforced by the microscopy in (B) and (C). On the left the sensor is in a low miRNA environment, and on the right the sensor is in a high miRNA environment.

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Categories
Parameters
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