Generator
pUS203

Part:BBa_K1388000

Designed by: Rokiah Alford, Tom Geddes, Andreas Bachler   Group: iGEM14_USyd-Australia   (2014-10-07)

IntI1 coding region under AraC-pBad control

This part is composed of IntI1 under control of the AraC-pBAD system from iGEM plasmid BBa_K731201.

It allows controlled expression of the IntI1 gene which produces integron Integrase. The integrase acts as a site-specific recombinase enzyme which allows insertion or excision of gene cassettes (with AttC sites) into appropriate recombination sites (AttI sites).

This part allows simple expression of Integrase with the well characterized AraC-pBAD promoter system. Integrase has a tendency to be toxic to the cell so it is recommended to move the part from the plasmid into a low copy plasmid.

Validation of this part is no completed. Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1312
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1223
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1058
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1557


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