IntI1 coding region under AraC-pBad control
This part is composed of IntI1 under control of the AraC-pBAD system from iGEM plasmid BBa_K731201.
It allows controlled expression of the IntI1 gene which produces integron Integrase. The integrase acts as a site-specific recombinase enzyme which allows insertion or excision of gene cassettes (with AttC sites) into appropriate recombination sites (AttI sites).
This part allows simple expression of Integrase with the well characterized AraC-pBAD promoter system. Integrase has a tendency to be toxic to the cell so it is recommended to move the part from the plasmid into a low copy plasmid.
Validation of this part is no completed. Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1312
- 21Illegal BamHI site found at 1223
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 1058
- 1000Illegal BsaI site found at 1557