Regulatory

Part:BBa_K1387004

Designed by: Tri Ekawati Heryanto   Group: iGEM14_ITB_Indonesia   (2014-10-07)

pIBPAB - B0034 - TetR - B0015

This casette is consist of inclusion body binding promoter which sensitively detect the formation of inclusion body. Then we combine this promoter to RBS, tetR, and double terminator so it will be more efficient. The construction functioned as self regulatory module. This module can produce tetR protein when inclusion body was formed and it will repress the expression of gene which have tetO as operator in the upstream of that gene, because tetR can bind to tetO.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None