Regulatory

Part:BBa_K1387003

Designed by: Tri Ekawati Heryanto   Group: iGEM14_ITB_Indonesia   (2014-10-07)

pIBPAB - B0034 - TetR

This casette is consist of inclusion body binding promoter which sensitively detect the formation of inclusion body in cell. Then combine this promoter to RBS, and tetR. When inclusion body form it will activated the promoter and tetR protein will poduced. Then tetR protein will bind at tetO (operator) and repress another gene. The construction functioned as self regulatory module.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None