Regulatory

Part:BBa_K1387002

Designed by: Tri Ekawati H, Joko Pebrianto T, Pande Putu Erawijantari, Nimas Ghasani, Gilang Tirta Widi   Group: iGEM14_ITB_Indonesia   (2014-09-29)

This construct consist of tetR (BBa_C0040) and double terminator (BBa_B0015). tetR is tetracycline repressor that bind to TetO operator region in degradation module and it can repressing the expression of downstream gene. In our system, the downstream gene is a fusion of lpp’-ompA-LC Cutinase. We combine tetR with double terminator B0015 so the transcription can more efficient, this is our self regulatory module.

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