Coding

Part:BBa_K1382001

Designed by: Maryam Ige, Janki Patel   Group: iGEM14_Stony_Brook   (2014-06-23)


GST-melittin

This consists of melittin fused to GST (Glutathione S-Tranferase) with a TEV cleavage site. Melittin is a toxin which causes cell death. GST-melittin is an inactive form of melittin, which ensures that melittin can be produced in a cell without killing the host cell. To activate melittin, TEV protease is added to cleave melittin from GST.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


[edit]
Categories
Parameters
None