Plasmid

Part:BBa_K138001

Designed by: Amrit Misra   Group: iGEM08_Michigan   (2008-10-12)


nifHp driving mutant NifL in Leucine Landing Pad

BBa_K138000 was cut with NdeI/SpeI and ligated with a mutant form of NifL digested with NdeI/SpeI. The mutant form of NifL binds and sequesters NifA with a higher affinity than the wild-type NifL does.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1209
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1209
    Illegal NotI site found at 973
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 364
    Illegal BglII site found at 955
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1209
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1209
    Illegal NgoMIV site found at 553
    Illegal NgoMIV site found at 657
    Illegal NgoMIV site found at 1275
    Illegal NgoMIV site found at 1472
    Illegal NgoMIV site found at 1770
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 663
    Illegal BsaI.rc site found at 1182
    Illegal SapI.rc site found at 1158


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