Generator
ATF1

Part:BBa_K1379008

Designed by: Nadia Benedicta Bongso   Group: iGEM14_Hong_Kong_HKUST   (2014-09-23)

Constitutive banana odor enzyme (ATF1) generator

This part is a composite part which contains constitutive promoter (BBa_J23102) expressing banana odor enzyme generator, BBa_J45219. The enzyme ATF1 catalyzes the conversion of isoamyl alcohol to odor isoamyl acetate which has the banana smell.

Usage and Biology

Supplement cultures with 5 Mm isoamyl alcohol to produce banana odor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 161
    Illegal BamHI site found at 1488
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 588
    Illegal SapI.rc site found at 1596


[edit]
Categories
//classic/generator/rct
//function/odor
Parameters
None