Device

Part:BBa_K1377001

Designed by: Jade Minseob Yoo   Group: iGEM14_Korea_U_Seoul   (2014-10-09)


spaA backward + sortase A + spaB + spaC

Pilin A gene cluster is coding for pili A of Corynebacterium diphtheriae. It has four coding regions and one regulatory region. Regulatory region is its own promoter. Four coding regions are sortase A, enzyme that makes pili A and pilin spa A & B & C that are components of pili A . WIth these all regions, we can make pili A. And we are going to modifying this part to generate "Our Favorite Protein Whip", especially spa A

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 416
    Illegal EcoRI site found at 4073
    Illegal EcoRI site found at 4250
    Illegal EcoRI site found at 6690
    Illegal EcoRI site found at 6777
    Illegal EcoRI site found at 6879
    Illegal PstI site found at 1642
    Illegal PstI site found at 3331
    Illegal PstI site found at 3649
    Illegal PstI site found at 7735
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 416
    Illegal EcoRI site found at 4073
    Illegal EcoRI site found at 4250
    Illegal EcoRI site found at 6690
    Illegal EcoRI site found at 6777
    Illegal EcoRI site found at 6879
    Illegal PstI site found at 1642
    Illegal PstI site found at 3331
    Illegal PstI site found at 3649
    Illegal PstI site found at 7735
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 416
    Illegal EcoRI site found at 4073
    Illegal EcoRI site found at 4250
    Illegal EcoRI site found at 6690
    Illegal EcoRI site found at 6777
    Illegal EcoRI site found at 6879
    Illegal BamHI site found at 97
    Illegal BamHI site found at 5858
    Illegal XhoI site found at 365
    Illegal XhoI site found at 1073
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 416
    Illegal EcoRI site found at 4073
    Illegal EcoRI site found at 4250
    Illegal EcoRI site found at 6690
    Illegal EcoRI site found at 6777
    Illegal EcoRI site found at 6879
    Illegal PstI site found at 1642
    Illegal PstI site found at 3331
    Illegal PstI site found at 3649
    Illegal PstI site found at 7735
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 416
    Illegal EcoRI site found at 4073
    Illegal EcoRI site found at 4250
    Illegal EcoRI site found at 6690
    Illegal EcoRI site found at 6777
    Illegal EcoRI site found at 6879
    Illegal PstI site found at 1642
    Illegal PstI site found at 3331
    Illegal PstI site found at 3649
    Illegal PstI site found at 7735
    Illegal NgoMIV site found at 401
    Illegal NgoMIV site found at 4458
    Illegal NgoMIV site found at 7397
    Illegal AgeI site found at 4941
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3432


[edit]
Categories
Parameters
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