Composite

Part:BBa_K1375018

Designed by: Zheng Xu   Group: iGEM14_SCU-China   (2014-10-10)

Prhl/las +RBS+GFP+DT+Pcon+RBS+LasR+RhlR+DT

This is a long part that produces GFP, regulated by C4-HSL and C12-HSL. We can make it express by adding C4-HSL, and reduce the expression by adding C12-HSL.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 953
    Illegal NheI site found at 976
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2034
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1329
    Illegal AgeI site found at 1526
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2509
    Illegal BsaI.rc site found at 725


[edit]
Categories
Parameters
None