Regulatory

Part:BBa_K137128

Designed by: Robert Ovadia   Group: iGEM08_Caltech   (2008-10-16)


B4 Lac Promoter - Tight expression levels

An additional lactose inducible promoter (similar to A81) obtained from: Cox RS III, Surette MG, Elowitz MB. Programming gene expression with combinatorial promoters. Mol. Syst. Biol. 2007;3:145. Used for tight expression levels to maintain our lysis cassette genes. This allows the cell to lyse only when lactose is present, as for lysis at any other time may cause problems in our system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 115
    Illegal XbaI site found at 5
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 116
    Illegal PstI site found at 130
    Illegal NotI site found at 123
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 116
    Illegal XbaI site found at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5
    Illegal SpeI site found at 116
    Illegal PstI site found at 130
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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