Project

Part:BBa_K137120

Designed by: Fei Chen   Group: iGEM08_Caltech   (2008-10-07)

LamB and TetA expression plasmid

Constitutively expresses the lamB gene, which codes for the lambda receptor. Following the lamB gene is the tetracycline resistance cassette.

Usage and Biology

The series of parts K137120-K137123 were placed in plasmid pSB1A2 and E. coli strain JW3996-1 (lamB::kanR). When infected with phage lambda, the cells produced the following relative plating efficiency (pfu/mL on engineered strain relative to pfu/mL on wild-type DH10B):

LamBTetA.gif

The plating efficiency correlates to expression level (determined using the promoter and RBS strengths listed in the Registry) with almost perfect linearity, suggesting that LamB expression is limiting infection frequency.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 213
    Illegal AgeI site found at 1274
  • 1000
    COMPATIBLE WITH RFC[1000]


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