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Part:BBa_K1371026

Designed by: Qifeng Tan   Group: iGEM14_SCUT-China   (2014-10-07)

Docking domain 6 pre

The fidelity and efficiency of acyl transfer on the interfaces of the individual PKS proteins is thought to be governed by helical regions, termed Docking Domains (DD), which are located at the C-terminus of the upstream and N-terminus of downstream polypeptide chains.

DD-fig1 .jpg

This part is KS-side docking domain (KS DD) at the N-terminus of the DD6. Each DD has formed specificity of the binding because it has specific connection site. Namely each docking domain is unique in the whole polyketone synthesis pathway. With this important feature, we can ensure the connection between adjacent modules does not have randomness, which makes the product unique.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 126
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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