Part:BBa_K1371005
L3-KR1
KR Part
The function of KR domain is deoxidizing the extended unit and enabling it to form the β-hydroxyl ester bond. In KR domain catalyzed reactions, the carbonyl groups of polyketide chains anchored on the ACP domains are combined with the specific binding sites of KR domains through weak chemical bonds. Then the electron cloud in the carbonyl groups is moving towards oxygen atoms so that the carbon atoms have net positive charge. At this time, NADPHs have a chance to bind to the polyketide chains and form instable ternary complexes. In order to reduce the inner energy of this ternary complexes and run towards stable, NADPHs provide hydride ions and KR domains provide protons to the complexes and convert the carbonyl groups to theβ-hydroxyl esters. Finally, the KR domain residues which have lost protons are deoxidized to the initial KR domains by other NADPHs.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 283
Illegal AgeI site found at 159
Illegal AgeI site found at 229
Illegal AgeI site found at 856
Illegal AgeI site found at 1138 - 1000COMPATIBLE WITH RFC[1000]
| None |