Composite

Part:BBa_K137078

Designed by: Doug Tischer   Group: iGEM08_Caltech   (2008-08-19)


AHL -> H2O2

This is the final construct from one of the projects of Caltech 2008 iGEM team. In response to being induced with acylhomoserine lactone, the construct should cause the E. coli to produce pyruvate oxidase (SpxB) while simulataneously shutting down production of a catalase, KatG. KatG is cleared rapidly from the cell due to an LAA degradation tag, allowing hydrogen peroxide produced from the pyruvate oxidase to build to bactericidal concentrations. Preliminary data suggests pyruvate oxidase can produce 0.8 mM hydrogen peroxide in the media supernatant.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4031
    Illegal BglII site found at 4966
    Illegal BglII site found at 5093
    Illegal BamHI site found at 2741
    Illegal BamHI site found at 4883
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005


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Categories
Parameters
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