Measurement

Part:BBa_K1369015

Designed by: Galen Gao   Group: iGEM14_Caltech   (2014-10-17)


fsrB_3xFLAG_N

Contains fsrB membrane protein from fsrABC quorum sensing system, just like BBa_K1369014, but adds 3xFLAG domain at the N-terminus (5' end) of the domain within fsrB responsible for coding GBAP (the signaling ligand in the fsrABC QS system). This serves as a target for antibody binding in Western blotting and purification columns. Available upon request from the Caltech 2014 iGEM team in a custom backbone.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 243
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 243
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 243
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 502


[edit]
Categories
Parameters
None