Measurement

Part:BBa_K1369013

Designed by: Galen Gao   Group: iGEM14_Caltech   (2014-10-17)


lamBD_3xFLAG_N

Same as part BBa_K1369012 (lamBD genes from Lactobacillus plantarum) but a 3xFLAG domain has been inserted at the N-terminus (5' end) of the lamD gene. This 3xFLAG sequence serves as a marker for antibody-binding in Western blotting and purification columns. Part available upon request from Caltech 2014 iGEM team in a custom backbone.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 99
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 99
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 258
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 99
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 99
    Illegal AgeI site found at 44
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None