Measurement
Part:BBa_K1369013
Designed by: Galen Gao Group: iGEM14_Caltech (2014-10-17)
lamBD_3xFLAG_N
Same as part BBa_K1369012 (lamBD genes from Lactobacillus plantarum) but a 3xFLAG domain has been inserted at the N-terminus (5' end) of the lamD gene. This 3xFLAG sequence serves as a marker for antibody-binding in Western blotting and purification columns. Part available upon request from Caltech 2014 iGEM team in a custom backbone.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 99
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 99
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 258
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 99
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 99
Illegal AgeI site found at 44 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |