Signalling

Part:BBa_K1369012

Designed by: Galen Gao   Group: iGEM14_Caltech   (2014-10-17)


lamBD

Contains lamB membrane protein and lamD signaling ligand prepeptide sequentially from 5' to 3'. These two components are needed for signaling ligand export for the lamBDCA quorum sensing system in Lactobacillus plantarum. Characterization by liquid chromatography/mass spectroscopy indicates this construct is nonfunctional in E. coli DH5a. Available from Caltech 2014 iGEM team in custom backbone.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 99
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 99
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 258
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 99
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 99
    Illegal AgeI site found at 44
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None