Part:BBa_K1369008

pCon FsrC, constituatively expressed non-inhibited HK with 4 SH3 domains

This plasmid contains FsrC, which is a histidine kinase used in E. coli. It has 4 SH3 domains, which lets the scaffold protein attach so that we can test the two component system without a ligand. In our project, we were designing a two-component system, with a histidine kinase and response regulator. If there is a change in the environment that the histidine kinase sense, the response regulator will act in response to it and express GFP.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1
  Illegal XbaI site found at 2693
  Illegal XbaI site found at 4719
  Illegal SpeI site found at 7
  Illegal PstI site found at 2699
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1
  Illegal NheI site found at 42
  Illegal NheI site found at 802
  Illegal SpeI site found at 7
  Illegal PstI site found at 2699
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1
  Illegal BglII site found at 80
  Illegal BamHI site found at 2300
  Illegal XhoI site found at 2309
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1
  Illegal XbaI site found at 2693
  Illegal XbaI site found at 4719
  Illegal SpeI site found at 7
  Illegal PstI site found at 2699
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1
  Illegal XbaI site found at 2693
  Illegal XbaI site found at 4719
  Illegal SpeI site found at 7
  Illegal PstI site found at 2699
  Illegal AgeI site found at 3963
  Illegal AgeI site found at 4287
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2408
  Illegal BsaI site found at 2586
  Illegal SapI.rc site found at 797
  Illegal SapI.rc site found at 1595
  Illegal SapI.rc site found at 1805
  Illegal SapI.rc site found at 2015
  Illegal SapI.rc site found at 2225