Plasmid
Part:BBa_K1369007
Designed by: Monique Alkiewicz Group: iGEM14_Caltech (2014-10-09)
pBAD FsrA-SH3pep, pFsrA-GFP, RR with direct recruitment (SH3 peptide). Ideal pBad concentrations wil
This plasmid contains FsrA, which is a response regulator used in E. coli. In our project, we were designing a two-component system, with a histidine kinase and response regulator. If there is a change in the environment that the histidine kinase sense, the response regulator will act in response to it and express GFP. This contains a scaffold protein, which allows the response regulator to react to the histidine kinase without a ligand.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 13
Illegal XbaI site found at 1648
Illegal XbaI site found at 3412
Illegal SpeI site found at 19
Illegal PstI site found at 4601 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 13
Illegal NheI site found at 1263
Illegal SpeI site found at 19
Illegal PstI site found at 4601
Illegal NotI site found at 5558 - 21INCOMPATIBLE WITH RFC[21]Illegal suffix found in sequence at 2105
Illegal EcoRI site found at 13
Illegal BglII site found at 1286 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 13
Illegal XbaI site found at 1648
Illegal XbaI site found at 3412
Illegal SpeI site found at 19
Illegal PstI site found at 4601 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 13
Illegal XbaI site found at 1648
Illegal XbaI site found at 3412
Illegal SpeI site found at 19
Illegal PstI site found at 4601
Illegal NgoMIV site found at 3867
Illegal NgoMIV site found at 4150
Illegal NgoMIV site found at 7832
Illegal AgeI site found at 1047
Illegal AgeI site found at 7992 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3127
Illegal BsaI site found at 3305
Illegal BsaI site found at 5238
Illegal BsaI.rc site found at 2963
Illegal SapI site found at 1029
Illegal SapI site found at 1616
Illegal SapI site found at 4090
Illegal SapI site found at 4300
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Categories
Parameters
None |