Plasmid

Part:BBa_K1369007

Designed by: Monique Alkiewicz   Group: iGEM14_Caltech   (2014-10-09)


pBAD FsrA-SH3pep, pFsrA-GFP, RR with direct recruitment (SH3 peptide). Ideal pBad concentrations wil

This plasmid contains FsrA, which is a response regulator used in E. coli. In our project, we were designing a two-component system, with a histidine kinase and response regulator. If there is a change in the environment that the histidine kinase sense, the response regulator will act in response to it and express GFP. This contains a scaffold protein, which allows the response regulator to react to the histidine kinase without a ligand.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 13
    Illegal XbaI site found at 1648
    Illegal XbaI site found at 3412
    Illegal SpeI site found at 19
    Illegal PstI site found at 4601
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 13
    Illegal NheI site found at 1263
    Illegal SpeI site found at 19
    Illegal PstI site found at 4601
    Illegal NotI site found at 5558
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2105
    Illegal EcoRI site found at 13
    Illegal BglII site found at 1286
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 13
    Illegal XbaI site found at 1648
    Illegal XbaI site found at 3412
    Illegal SpeI site found at 19
    Illegal PstI site found at 4601
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 13
    Illegal XbaI site found at 1648
    Illegal XbaI site found at 3412
    Illegal SpeI site found at 19
    Illegal PstI site found at 4601
    Illegal NgoMIV site found at 3867
    Illegal NgoMIV site found at 4150
    Illegal NgoMIV site found at 7832
    Illegal AgeI site found at 1047
    Illegal AgeI site found at 7992
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3127
    Illegal BsaI site found at 3305
    Illegal BsaI site found at 5238
    Illegal BsaI.rc site found at 2963
    Illegal SapI site found at 1029
    Illegal SapI site found at 1616
    Illegal SapI site found at 4090
    Illegal SapI site found at 4300


[edit]
Categories
Parameters
None